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Journal: bioRxiv

Article Title: Molecular Star Gazing: Development and Validation of an Environmental DNA Assay for the Imperiled Sunflower Sea Star ( Pycnopodia helianthoides )

doi: 10.64898/2026.05.07.723600

Figure Lengend Snippet:

Article Snippet: Nucleic-acid extracts were run as template (5 μl) in three replicate ddPCR reactions using the finalized ddPCR conditions (see Results) and an AutoDG Automated Droplet Generator (Bio-Rad, Hercules, CA), a C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA) and a QX200 Droplet Digital PCR System (Bio-Rad, Hercules, CA) ( ).

Techniques:

Proportion of samples or dive transects across the four regions with a positive detection of Pycnopodia by either underwater visual census (UVC) or by ddPCR assay of water samples (eDNA). Samples below LoD were set to zero.

Journal: bioRxiv

Article Title: Molecular Star Gazing: Development and Validation of an Environmental DNA Assay for the Imperiled Sunflower Sea Star ( Pycnopodia helianthoides )

doi: 10.64898/2026.05.07.723600

Figure Lengend Snippet: Proportion of samples or dive transects across the four regions with a positive detection of Pycnopodia by either underwater visual census (UVC) or by ddPCR assay of water samples (eDNA). Samples below LoD were set to zero.

Article Snippet: Nucleic-acid extracts were run as template (5 μl) in three replicate ddPCR reactions using the finalized ddPCR conditions (see Results) and an AutoDG Automated Droplet Generator (Bio-Rad, Hercules, CA), a C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA) and a QX200 Droplet Digital PCR System (Bio-Rad, Hercules, CA) ( ).

Techniques:

eDNA concentrations measured by ddPCR assay after log10 transformation. Concentrations below the limit of detection were set to zero. Pycnopodia helianthoides biomass density at the transect level was calculated as kg per 10 m2 using a length–biomass conversion from . We find better alignment in eDNA and diver abundance estimates when averaging at larger scales as compared to point transect and bottle comparisons. Fjords are depicted in purple and Outer Islands are in orange.

Journal: bioRxiv

Article Title: Molecular Star Gazing: Development and Validation of an Environmental DNA Assay for the Imperiled Sunflower Sea Star ( Pycnopodia helianthoides )

doi: 10.64898/2026.05.07.723600

Figure Lengend Snippet: eDNA concentrations measured by ddPCR assay after log10 transformation. Concentrations below the limit of detection were set to zero. Pycnopodia helianthoides biomass density at the transect level was calculated as kg per 10 m2 using a length–biomass conversion from . We find better alignment in eDNA and diver abundance estimates when averaging at larger scales as compared to point transect and bottle comparisons. Fjords are depicted in purple and Outer Islands are in orange.

Article Snippet: Nucleic-acid extracts were run as template (5 μl) in three replicate ddPCR reactions using the finalized ddPCR conditions (see Results) and an AutoDG Automated Droplet Generator (Bio-Rad, Hercules, CA), a C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA) and a QX200 Droplet Digital PCR System (Bio-Rad, Hercules, CA) ( ).

Techniques: Transformation Assay